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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has actually been noticed in several tumors, which ends up in chromosome instability, and so may perhaps lead to tumorigenesis. This protein was located to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant purpose in endorsing mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two additional splice variants ended up noted but detailed nucleotide sequence data isn't nevertheless obtainable.

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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged because of the antibody.

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This guideline outlines further more doable optimization of cellular and immunoassay parameters to be sure the best possible final results are...

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This information outlines even more doable optimization of mobile and immunoassay parameters to make certain the best possible results are...

Antibodies sure to the blocking peptide no longer bind on the epitope within the concentrate on protein. By evaluating the staining within the blocked antibody vs . the antibody by itself, you can see which staining is specific.

Target Info Cyclin E belongs towards the extremely conserved cyclin spouse and children, whose members are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a fancy with and functions as a regulatory subunit of CDK2, whose action is required for cell cycle G1/S changeover. This protein accumulates in the G1-S stage boundary and it is degraded as cells development through S phase.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...

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